Shimoji s selective enrichment broth contains tryptic soy broth, Tween 80, Tris-aminomethane, crystal violet and sodium azide.
2.
However, this method ( bypassing the selective enrichment broth step ) can lead to some false-negative results, and this approach should be taken only in addition to, and not instead of, inoculation into selective broth.
3.
To increase recovery of GBS, swabs can also be inoculated previously into a selective enrichment broth medium, such as the Todd-Hewitt broth supplemented with gentamicin or colistin and nalidixic acid and incubated for 18 24 hours at 35-37 �C.
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